Reporter

Part:BBa_K3183028:Design

Designed by: Ferenc Daniel Felfoldi   Group: iGEM19_Oxford   (2019-10-21)


erm-mClover3 codon optimized for L. reuteri


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

-


Source

This part is synthesized from P-erm promoter and mClover3. mClover3 was first described by Bajar et al. 2016. P-erm promoter is the erythromycin ribosomal methylase (ermB) promoter from the broad-host range plasmid pAMβ1 isolated from Enterococcus faecalis. We ordered the sequences as a part of a gBlock from IDT nand has been assembled with Gibson Assembly.

References